12/12/2023 0 Comments Macrophages in synovial fluid![]() Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors. , Bonaguro Lorenzo, Gemünd Ioanna, Reusch Nico, Saglam Adem, Hinkley Emily R. Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases. 13.įibroblast growth factor 23 and bone mineralisation, Int J Oral Sci, № 7, с. GATA6+ Peritoneal Resident Macrophage: The Immune Custodian in the Peritoneal Cavity. Matrix metalloproteinases in bone development and pathology: current knowledge and potential clinical utility, Metalloproteinases In Medicine, № 3, с. 16009Ĭlassical and Paradoxical Effects of TNF-α on Bone Homeostasis, Front Immunol, № 13, с. TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease, Bone Res, № 26, с. Role of transforming growth factor-beta in bone remodeling, Clin Orthop Relat Res, № 250, с. Interferon-Gamma-Mediated Osteoimmunology, Front Immunol, № 29, с. ![]() 11.īiology of RANK, RANKL, and osteoprotegerin. Coelho Inês, Duarte Nádia, Barros André, Macedo Maria Paula, Penha-Gonçalves Carlos. Trem-2 Promotes Emergence of Restorative Macrophages and Endothelial Cells During Recovery From Hepatic Tissue Damage. EnhancedVolcano: Publication-ready volcano plots with enhanced colouring and labeling. 2020, bioRxiv.īlighe K, Rana S, Lewis M. Griss J, Viteri G, Sidiropoulos K, Nguyen V, Fabregat A, Hermjakob H. ReactomeGSA - Efficient Multi-Omics Comparative Pathway Analysis. Hao Y, Hao S, Andersen-Nissen E, III WMM, Zheng S, Butler A, Lee MJ, Wilk AJ, Darby C, Zagar M, Hoffman P, Stoeckius M, Papalexi E, Mimitou EP, Jain J, Srivastava A, Stuart T, Fleming LB, Yeung B, Rogers AJ, McElrath JM, Blish CA, Gottardo R, et al. Integrated analysis of multimodal single-cell data. Transwell Permeable Supports Selection and Use Guide. 2021, Front Immunol.Ĭorning Incorporated. 1351Ĭritical Role of Synovial Tissue-Resident Macrophage and Fibroblast Subsets in the Persistence of Joint Inflammation. High percentages and activity of synovial fluid NK cells present in patients with advanced stage active Rheumatoid Arthritis, Sci Rep, № 9, с. Origin and function of synovial macrophage subsets during inflammatory joint disease, Adv Immunol, № 143, с. RSM analysis via scRNA-seq indicated these cells are M2 skewed, capable of antigen presentation, and have consistent functions in both septic and inflammatory arthritis. scRNA-seq revealed a population consistent with RSM transcriptionally related to CD56+cytotoxic dendritic cells and IDO+M2 macrophages.ConclusionWe identified a rare cell population consistent with RSM, indicating these cells are likely migratory and able to initiate or coordinate both acute (septic) or chronic (autoimmune or inflammatory) arthritis. Similar putative RSM were identified usingex vivomigration assays when MCP-1 and LPS were used as migratory stimulus. These cells were relatively enriched in the SF during infectious processes, but absolutely decreased compared to healthy controls. ELISA was used to evaluate the bone-resorption potential of SF.ResultsWe were able to identify a rare population of CD14dim, OPG+, ZO-1+cells consistent with RSM in SF via flow cytometry. We used single cell RNA-sequencing (scRNA-seq) to further identify macrophage/monocyte subsets. We used a novel transwell migration assay with humanex-vivosynovium obtained intra-operatively to validate flow cytometry findings. We used flow cytometry to identify mononuclear cells in peripheral blood and SF. We sought to identify RSM in synovial fluid (SF) and demonstrate migratory ability, in additional to functional changes that may perpetuate a chronic inflammatory response within joint spaces.MethodsWe recruited human patients presenting with undifferentiated arthritis in multiple clinical settings. AbstractObjectivesResident synovial macrophages (RSM) provide immune sequestration of the joint space and are likely involved in initiation and perpetuation of the joint-specific immune response.
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